I-3 Hypothermic low flow perfusion method

Regional low-flow perfusion improves neurologic outcome compared with deep hypothermic circulatory arrest in neonatal piglets.

BACKGROUND Regional low-flow perfusion is an alternative to deep hypothermic circulatory arrest, but whether regional low-flow perfusion improves neurologic outcome after deep hypothermic circulatory arrest in neonates remains unknown. We tested neurologic recovery after regional low-flow perfusion compared with deep hypothermic circulatory arrest in a neonatal piglet model. METHODS Sixteen n...

The technique of hypothermic perfusion.

The machine described is in commercial production although the perfusions, to date, were performed with the prototype described by Ross (1960a). The extracorporeal circuit is mounted on the front of the machine (Fig. 1) under the vision of the perfusionist and comprises: Coronary suction reservoir (A) into which all wound and coronary sinus blood is returned by suction from a horizontally mount...

Hypothermic Machine Perfusion of Composite Tissues

Flow and Contractile Function of Isolated Hearts After Prolonged Hypothermic Perfusion

Background Normal ionic perfusate containing butanedione monoxime (BDM), a reversible myofilament inhibitor, could be better than either a high potassium (KCl) or a low calcium (CaCl2) perfusate for long-term cardiac preservation. This hypothesis was tested in 70 isolated guinea pig hearts. Methods and Results Three groups time control (8 hours, 370C), cold control (22 hours, 3.80C), and cold+B...

Liver preservation by single passage hypothermic "squirt" perfusion.

A single passage, hypothermic, intermittent squirt perfusion of the pig's liver is described which has allowed preservation of porcine livers for up to 17 hours. After preservation, orthotopically allografted livers can maintain recipient animals in good health. If this technique can be applied to clinical liver transplantation it should be possible to move livers from one hospital to another i...